Proanthocyanidins (PCs) have shown inhibition of oxidative damage by improving Nrf-2 expression inmany tissues. However, the\ncytoprotective effects of PCs onH2O2-induced tendon damage have not been verified. The current study was aimed at assessing the\ncytoprotection of PCs on the oxidative cellular toxicity of tendon-derived stem cells (TDSCs) induced by H2O2. The TDSCs were\nisolated frompatellar tendons of Sprague Dawley (SD) rats, and the cells after third passage were used for subsequent experiments.\nThe isolated cells were identified by flow cytometry assay and multidifferentiation potential assay. Cell Counting Kit-8 assay was\nperformed to examine cell viability. Real-Time PCR andWestern Blot were employed to, respectively, assess the mRNA and protein\nexpressions of Nrf-2, GCLM, NQO-1, and HO-1. PCs significantly improved the cell viability of TDSCs. Furthermore, H2O2\nupregulated Nrf-2, GCLM, NQO-1, and HO-1 without significant difference, while the proteins expressions were increased with\nsignificant difference in PCs group and PCs + H2O2 cotreated group. All the findings indicated that PCs could protect against\nthe oxidative damage induced by H2O2 in TDSCs, and the cytoprotective effects might be due to the ability of PCs to activate the\nexpressions of GCLM, HO-1, and NQO-1 via upregulating Nrf-2 signaling pathway.
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